6LUR

Human PUF60 UHM domain (thioredoxin fusion) in complex with a small molecule binder

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Thioredoxin 1,Poly(U)-binding-splicing factor PUF60	polymer	222	24671.9	8	UniProt (P0AA25) UniProt (Q9UHX1) Pfam (PF00085)	Escherichia coli (strain K12)	Trx-1,60 kDa poly(U)-binding-splicing factor,FUSE-binding protein-interacting repressor,FBP-interacting repressor,Ro-binding protein 1,RoBP1,Siah-binding protein 1,Siah-BP1
2	I, J, K, L, M... (B, C, D, E)	4-[2-[4-(aminomethyl)phenyl]phenyl]piperazin-2-one	non-polymer		281.4	8	Chemie (EVU)
3	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	696	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 344 - 452 (UniProt: P0AA25)

PDB	External Database	Details
Met 335	-	initiating methionine
Lys 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
Pro 343	-	expression tag
Gly 453	-	linker
Ser 454	-	linker
Ala 455	-	linker
Met 456	-	linker

A, B, C, D, E, F, G, H: 457 - 556 (UniProt: Q9UHX1)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	197375.4
Non-Polymers*	Number of molecules	8
	Total formula weight	2250.8
All*	Total formula weight	199626.3

*Water molecules are not included.