6LKN

Crystal structure of ATP11C-CDC50A in PtdSer-bound E2P state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, E, I, M)	Phospholipid-transporting ATPase IG	polymer	1129	129710.6	4	UniProt (Q8NB49) Pfam (PF16209) Pfam (PF00122) Pfam (PF13246) Pfam (PF16212)	Homo sapiens (Human)	ATPase IQ,ATPase class VI type 11C,P4-ATPase flippase complex alpha subunit ATP11C
2	B, D, F, H (C, F, J, N)	Cell cycle control protein 50A	polymer	361	40840.7	4	UniProt (Q9NV96) Pfam (PF03381)	Homo sapiens (Human)	P4-ATPase flippase complex beta subunit TMEM30A,Transmembrane protein 30A
3	GA, I, K, Q, R... (M, A, C, E, I)	O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine	non-polymer		792.1	7	Chemie (P5S)
4	AA, HA, J, S (I, M, A, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	BA, CA, EA, FA, IA... (J, N, C, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	16	Chemie (NAG)
6	DA, KA, N, V (J, N, C, F)	1-deoxy-alpha-D-mannopyranose	non-polymer		164.2	4	Chemie (AH2)
7	NA, OA, PA, QA (A, E, I, M)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, E, I, M: 10 - 1129 (UniProt: Q8NB49)

PDB	External Database	Details
Met 1	-	initiating methionine
Phe 2	-	expression tag
Arg 3	-	expression tag
Arg 4	-	expression tag
Ser 5	-	expression tag
Leu 6	-	expression tag
Asn 7	-	expression tag
Arg 8	-	expression tag
Phe 9	-	expression tag
Trp 111	Cys 114	engineered mutation

C, F, J, N: 1 - 361 (UniProt: Q9NV96)

PDB	External Database	Details
Gln 190	Asn 190	engineered mutation
Trp 292	Ser 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	682205.3
Non-Polymers*	Number of molecules	31
	Total formula weight	9837.7
All*	Total formula weight	692043.0

*Water molecules are not included.