6LKN
Crystal structure of ATP11C-CDC50A in PtdSer-bound E2P state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-04-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.460, 232.830, 492.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.970 - 3.900 |
| R-factor | 0.2846 |
| Rwork | 0.281 |
| R-free | 0.35010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k7l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 1.141 | |
| Rpim | 0.042 | |
| Number of reflections | 106458 | 1223 |
| <I/σ(I)> | 15.88 | |
| Completeness [%] | 71.3 | 11.64 |
| Redundancy | 776.9 | 698.5 |
| CC(1/2) | 0.920 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 14-15% PEG4000, 10% glycerol, 5 mM beta-mercaptoethanol, 0.4 M MgSO4 |
