6I5T

Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (F, E, D, C, B...)	Bifunctional nitrilase/nitrile hydratase NIT4	polymer	361	39766.1	6	UniProt (P46011) Pfam (PF00795)	Arabidopsis thaliana (Mouse-ear cress)	Cyanoalanine nitrilase,Nitrilase 4

Sequence modifications

F, E, D, C, B, A: 1 - 355 (UniProt: P46011)

PDB	External Database	Details
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	238596.7
All*	Total formula weight	238596.7

*Water molecules are not included.