6I5T

Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000257	molecular_function	nitrilase activity
A	0003824	molecular_function	catalytic activity
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0018822	molecular_function	nitrile hydratase activity
A	0019499	biological_process	cyanide metabolic process
A	0047427	molecular_function	cyanoalanine nitrilase activity
A	0047558	molecular_function	3-cyanoalanine hydratase activity
A	0051410	biological_process	detoxification of nitrogen compound
A	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
B	0000257	molecular_function	nitrilase activity
B	0003824	molecular_function	catalytic activity
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0018822	molecular_function	nitrile hydratase activity
B	0019499	biological_process	cyanide metabolic process
B	0047427	molecular_function	cyanoalanine nitrilase activity
B	0047558	molecular_function	3-cyanoalanine hydratase activity
B	0051410	biological_process	detoxification of nitrogen compound
B	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
C	0000257	molecular_function	nitrilase activity
C	0003824	molecular_function	catalytic activity
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0018822	molecular_function	nitrile hydratase activity
C	0019499	biological_process	cyanide metabolic process
C	0047427	molecular_function	cyanoalanine nitrilase activity
C	0047558	molecular_function	3-cyanoalanine hydratase activity
C	0051410	biological_process	detoxification of nitrogen compound
C	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
D	0000257	molecular_function	nitrilase activity
D	0003824	molecular_function	catalytic activity
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0016787	molecular_function	hydrolase activity
D	0016829	molecular_function	lyase activity
D	0018822	molecular_function	nitrile hydratase activity
D	0019499	biological_process	cyanide metabolic process
D	0047427	molecular_function	cyanoalanine nitrilase activity
D	0047558	molecular_function	3-cyanoalanine hydratase activity
D	0051410	biological_process	detoxification of nitrogen compound
D	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
E	0000257	molecular_function	nitrilase activity
E	0003824	molecular_function	catalytic activity
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0016787	molecular_function	hydrolase activity
E	0016829	molecular_function	lyase activity
E	0018822	molecular_function	nitrile hydratase activity
E	0019499	biological_process	cyanide metabolic process
E	0047427	molecular_function	cyanoalanine nitrilase activity
E	0047558	molecular_function	3-cyanoalanine hydratase activity
E	0051410	biological_process	detoxification of nitrogen compound
E	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
F	0000257	molecular_function	nitrilase activity
F	0003824	molecular_function	catalytic activity
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0016787	molecular_function	hydrolase activity
F	0016829	molecular_function	lyase activity
F	0018822	molecular_function	nitrile hydratase activity
F	0019499	biological_process	cyanide metabolic process
F	0047427	molecular_function	cyanoalanine nitrilase activity
F	0047558	molecular_function	3-cyanoalanine hydratase activity
F	0051410	biological_process	detoxification of nitrogen compound
F	0080061	molecular_function	indole-3-acetonitrile nitrilase activity

Functional Information from PROSITE/UniProt

site_id	PS00920
Number of Residues	16
Details	NITRIL_CHT_1 Nitrilases / cyanide hydratase signature 1. GsqLVvFpEafIgGYP

Chain	Residue	Details
F	GLY68-PRO83

---

site_id	PS00921
Number of Residues	14
Details	NITRIL_CHT_2 Nitrilases / cyanide hydratase active site signature. GAaiCWENrmPSlR

Chain	Residue	Details
F	GLY193-ARG206

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10105","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---