6I5T
Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.9 |
| Number of Particle | 133106 |
| Software | RELION,EPU,Gctf,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
