6H2V

Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 2.5A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Methyltransferase-like protein 5	polymer	215	24581.4	2	UniProt (Q9NRN9) Pfam (PF05175) In PDB	Homo sapiens (Human)
2	B, D	Multifunctional methyltransferase subunit TRM112-like protein	polymer	125	14291.5	2	UniProt (Q9UI30) Pfam (PF03966) In PDB	Homo sapiens (Human)	tRNA methyltransferase 112 homolog
3	A, C	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
4	A, B, C, D	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
5	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	B, D	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	C, D	2-(2-METHOXYETHOXY)ETHANOL	non-polymer		120.1	2	Chemie (PG0)
8		water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, C: 1 - 209 (UniProt: Q9NRN9)

PDB	External Database	Details
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	77745.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1939.9
All*	Total formula weight	79685.7

*Water molecules are not included.