6H2V

Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 2.5A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97996
Spacegroup name	P 21 21 21
Unit cell lengths	56.380, 105.894, 169.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.770 - 2.490
R-factor	0.215
Rwork	0.213
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h2u
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.490	2.490
Rmerge	0.144
Number of reflections	36060
<I/σ(I)>	14.05
Completeness [%]	99.8	99
Redundancy	13.5
CC(1/2)	0.999	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	25% PEG 4,000; 0.2 M ammonium sulfate; 100 mM Na citrate pH 5.6