6H2V

Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 2.5A resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001650	cellular_component	fibrillar center
A	0003676	molecular_function	nucleic acid binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005730	cellular_component	nucleolus
A	0005829	cellular_component	cytosol
A	0006364	biological_process	rRNA processing
A	0008168	molecular_function	methyltransferase activity
A	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
A	0031167	biological_process	rRNA methylation
A	0032259	biological_process	methylation
A	0042995	cellular_component	cell projection
A	0045202	cellular_component	synapse
A	0045727	biological_process	positive regulation of translation
A	0048863	biological_process	stem cell differentiation
A	0098793	cellular_component	presynapse
A	0098794	cellular_component	postsynapse
A	1904047	molecular_function	S-adenosyl-L-methionine binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006364	biological_process	rRNA processing
B	0008276	molecular_function	protein methyltransferase activity
B	0018364	biological_process	peptidyl-glutamine methylation
B	0030488	biological_process	tRNA methylation
B	0031167	biological_process	rRNA methylation
B	0032991	cellular_component	protein-containing complex
B	0045815	biological_process	transcription initiation-coupled chromatin remodeling
B	0046982	molecular_function	protein heterodimerization activity
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0070476	biological_process	rRNA (guanine-N7)-methylation
B	0141106	molecular_function	tRNA methyltransferase activator activity
B	2000234	biological_process	positive regulation of rRNA processing
C	0001650	cellular_component	fibrillar center
C	0003676	molecular_function	nucleic acid binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005730	cellular_component	nucleolus
C	0005829	cellular_component	cytosol
C	0006364	biological_process	rRNA processing
C	0008168	molecular_function	methyltransferase activity
C	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
C	0031167	biological_process	rRNA methylation
C	0032259	biological_process	methylation
C	0042995	cellular_component	cell projection
C	0045202	cellular_component	synapse
C	0045727	biological_process	positive regulation of translation
C	0048863	biological_process	stem cell differentiation
C	0098793	cellular_component	presynapse
C	0098794	cellular_component	postsynapse
C	1904047	molecular_function	S-adenosyl-L-methionine binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006364	biological_process	rRNA processing
D	0008276	molecular_function	protein methyltransferase activity
D	0018364	biological_process	peptidyl-glutamine methylation
D	0030488	biological_process	tRNA methylation
D	0031167	biological_process	rRNA methylation
D	0032991	cellular_component	protein-containing complex
D	0045815	biological_process	transcription initiation-coupled chromatin remodeling
D	0046982	molecular_function	protein heterodimerization activity
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0070476	biological_process	rRNA (guanine-N7)-methylation
D	0141106	molecular_function	tRNA methyltransferase activator activity
D	2000234	biological_process	positive regulation of rRNA processing

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAM A 301

Chain	Residue
A	PHE19
A	GLY61
A	CYS62
A	VAL64
A	ASP81
A	ILE82
A	CYS107
A	ASP108
A	VAL109
A	ASN126
A	PRO128
A	LEU26
A	HOH401
A	GLU27
A	GLN28
A	TYR29
A	PRO30
A	THR31
A	GLY59
A	CYS60

---

site_id	AC2
Number of Residues	5
Details	binding site for residue SO4 A 302

Chain	Residue
A	THR151
A	LYS175
A	ARG206
A	SER208
A	HIS211

---

site_id	AC3
Number of Residues	3
Details	binding site for residue SO4 A 303

Chain	Residue
A	LYS175
A	HIS210
A	HIS211

---

site_id	AC4
Number of Residues	3
Details	binding site for residue SO4 A 304

Chain	Residue
A	ARG32
A	PRO187
A	ALA188

---

site_id	AC5
Number of Residues	6
Details	binding site for residue PEG B 201

Chain	Residue
A	GLU84
A	GLN106
B	PRO108
B	SER110
B	ILE113
B	ASN115

---

site_id	AC6
Number of Residues	1
Details	binding site for residue EDO B 202

Chain	Residue
B	GLU90

---

site_id	AC7
Number of Residues	3
Details	binding site for residue SO4 B 203

Chain	Residue
B	GLU36
B	PHE37
B	ARG82

---

site_id	AC8
Number of Residues	20
Details	binding site for residue SAM C 301

Chain	Residue
C	PHE19
C	LEU26
C	GLU27
C	GLN28
C	TYR29
C	PRO30
C	THR31
C	GLY59
C	CYS60
C	GLY61
C	CYS62
C	VAL64
C	ASP81
C	ILE82
C	CYS107
C	ASP108
C	VAL109
C	ASN126
C	PRO128
C	HOH401

---

site_id	AC9
Number of Residues	5
Details	binding site for residue PG0 C 302

Chain	Residue
A	LYS132
A	ASN133
C	ARG32
C	PRO33
C	HIS34

---

site_id	AD1
Number of Residues	4
Details	binding site for residue SO4 C 303

Chain	Residue
C	THR151
C	ARG206
C	SER208
C	HIS211

---

site_id	AD2
Number of Residues	3
Details	binding site for residue SO4 C 304

Chain	Residue
C	LYS175
C	HIS210
C	HIS211

---

site_id	AD3
Number of Residues	8
Details	binding site for residue EDO D 201

Chain	Residue
C	ASN91
C	ASP103
C	MET104
D	CME33
D	GLU92
D	ARG111
D	GLY112
D	PG0202

---

site_id	AD4
Number of Residues	8
Details	binding site for residue PG0 D 202

Chain	Residue
C	GLU95
D	GLU29
D	ARG31
D	CME33
D	ILE94
D	GLU95
D	ARG111
D	EDO201

---

site_id	AD5
Number of Residues	3
Details	binding site for residue SO4 D 203

Chain	Residue
B	ARG62
D	PHE37
D	ARG82

---

Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. VIMNPPF

Chain	Residue	Details
A	VAL123-PHE129

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231

Chain	Residue	Details
B	SER119
C	ASP108
D	SER119
A	GLY59
A	ASP81
A	ASP108
C	GLN28
C	THR31
C	GLY59
C	ASP81

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
B	SER125
D	SER125

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:31328227, ECO:0007744|PDB:6H2V

Chain	Residue	Details
A	VAL64
C	VAL64

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