6FC1

Crystal structure of the eIF4E-Eap1p complex from Saccharomyces cerevisiae in the cap-bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Eukaryotic translation initiation factor 4E	polymer	193	21822.4	2	UniProt (P07260) Pfam (PF01652)	Saccharomyces cerevisiae S288C (Baker's yeast)	eIF4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein
2	B, D (B, D)	Protein EAP1	polymer	64	7590.8	2	UniProt (P36041)	Saccharomyces cerevisiae S288C (Baker's yeast)	eIF4E-associated protein 1
3	E, H (A, C)	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		538.2	2	Chemie (MGP)
4	F, G, I, J, K... (A, B, C, D)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
5	M, N, O, P (A, B, C, D)	water	water		18.0	514	Chemie (HOH)

Sequence modifications

A, C: 25 - 213 (UniProt: P07260)

PDB	External Database	Details
Gly 21	-	expression tag
Pro 22	-	expression tag
His 23	-	expression tag
Met 24	-	expression tag
Ala 42	Lys 42	conflict
Ala 122	Lys 122	conflict
Ala 168	Lys 168	conflict
Ala 187	Lys 187	conflict

B, D: 91 - 150 (UniProt: P36041)

PDB	External Database	Details
Gly 87	-	expression tag
Pro 88	-	expression tag
His 89	-	expression tag
Met 90	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	58826.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1629.0
All*	Total formula weight	60455.3

*Water molecules are not included.