6FC1
Crystal structure of the eIF4E-Eap1p complex from Saccharomyces cerevisiae in the cap-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99996 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.712, 121.726, 62.348 |
| Unit cell angles | 90.00, 132.02, 90.00 |
Refinement procedure
| Resolution | 46.317 - 1.350 |
| R-factor | 0.1392 |
| Rwork | 0.138 |
| R-free | 0.16830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fbz chain A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 108809 | 5058 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 98.1 | 92.6 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MIB (sodium malonate:imidazole:boric acid in molar ratios of 2:3:3) buffer (pH 7.0) 21% (w/v) PEG 1500 |
