6DXW

Human N-acylethanolamine-hydrolyzing acid amidase (NAAA) precursor (C126A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	N-acylethanolamine-hydrolyzing acid amidase	polymer	341	38571.9	4	UniProt (Q02083) Pfam (PF15508) Pfam (PF02275) In PDB	Homo sapiens (Human)	Acid ceramidase-like protein,N-acylsphingosine amidohydrolase-like,ASAH-like protein
2	E, G	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	F	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
4	H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
5	C, A, D, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
6	B, C, A, D	CHLORIDE ION	non-polymer		35.5	28	Chemie (CL)
7		water	water		18.0	856	Chemie (HOH)

Sequence modifications

A, B, C, D: 29 - 359 (UniProt: Q02083)

PDB	External Database	Details
Asp 19	-	expression tag
Arg 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
Lys 27	-	expression tag
Leu 28	-	expression tag
Ala 113	Cys 113	conflict
Ala 126	Cys 126	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154287.8
Branched	Number of molecules	4
	Total formula weight	1786.7
Non-Polymers*	Number of molecules	33
	Total formula weight	2098.7
All*	Total formula weight	158173.2

*Water molecules are not included.