6DXW
Human N-acylethanolamine-hydrolyzing acid amidase (NAAA) precursor (C126A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-17 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.909, 140.011, 90.123 |
Unit cell angles | 90.00, 115.20, 90.00 |
Refinement procedure
Resolution | 38.257 - 2.300 |
R-factor | 0.1679 |
Rwork | 0.167 |
R-free | 0.19330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u7z |
RMSD bond length | 0.005 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 87300 | |
<I/σ(I)> | 18.5 | |
Completeness [%] | 100.0 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1 M LiCl, 0.1 M MES pH 6 and 10 % PEG 6000 |