6D6Q

Human nuclear exosome-MTR4 RNA complex - overall reconstruction

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Exosome complex component RRP45	polymer	473	52828.0	1	UniProt (Q06265) Pfam (PF01138) Pfam (PF03725) In PDB	Homo sapiens (Human)	EXOSC9,Autoantigen PM/Scl 1,Exosome component 9,P75 polymyositis-scleroderma overlap syndrome-associated autoantigen,Polymyositis/scleroderma autoantigen 1,Polymyositis/scleroderma autoantigen 75 kDa,PM/Scl-75
2	B	Exosome complex component RRP41	polymer	249	26831.5	1	UniProt (Q9NPD3) Pfam (PF01138) Pfam (PF03725) In PDB	Homo sapiens (Human)	EXOSC4,Exosome component 4,Ribosomal RNA-processing protein 41,p12A
3	C	Exosome complex component RRP43	polymer	278	30285.8	1	UniProt (Q96B26) Pfam (PF01138) Pfam (PF03725) In PDB	Homo sapiens (Human)	EXOSC8,Exosome component 8,Opa-interacting protein 2,OIP-2,Ribosomal RNA-processing protein 43,p9
4	D	Exosome complex component RRP46	polymer	237	25480.2	1	UniProt (Q9NQT4) Pfam (PF01138) Pfam (PF03725) In PDB	Homo sapiens (Human)	EXOSC5,Chronic myelogenous leukemia tumor antigen 28,Exosome component 5,Ribosomal RNA-processing protein 46,p12B
5	E	Exosome complex component RRP42	polymer	293	32072.6	1	UniProt (Q15024) Pfam (PF03725) Pfam (PF01138) In PDB	Homo sapiens (Human)	EXOSC7,Exosome component 7,Ribosomal RNA-processing protein 42,p8
6	F	Exosome complex component MTR3	polymer	272	28267.1	1	UniProt (Q5RKV6) Pfam (PF01138) In PDB	Homo sapiens (Human)	EXOSC6,Exosome component 6,mRNA transport regulator 3 homolog,hMtr3,p11
7	G	Exosome complex component RRP40	polymer	277	29796.4	1	UniProt (Q9NQT5) Pfam (PF21261) Pfam (PF21262) Pfam (PF15985) In PDB	Homo sapiens (Human)	EXOSC3,Exosome component 3,Ribosomal RNA-processing protein 40,p10
8	H	Exosome complex component RRP4	polymer	296	33184.4	1	UniProt (Q13868) Pfam (PF14382) Pfam (PF21266) Pfam (PF15985) In PDB	Homo sapiens (Human)	EXOSC2,Exosome component 2,Ribosomal RNA-processing protein 4
9	I	Exosome complex component CSL4	polymer	197	21691.0	1	UniProt (Q9Y3B2) Pfam (PF14382) Pfam (PF10447) In PDB	Homo sapiens (Human)	EXOSC1,Exosome component 1
10	J	Exosome component 10	polymer	761	86754.9	1	UniProt (Q01780) Pfam (PF08066) Pfam (PF01612) Pfam (PF00570) In PDB	Homo sapiens (Human)	EXOSC10,Autoantigen PM/Scl 2,P100 polymyositis-scleroderma overlap syndrome-associated autoantigen,Polymyositis/scleroderma autoantigen 100 kDa,PM/Scl-100,Polymyositis/scleroderma autoantigen 2
11	K	Exosome complex exonuclease RRP44	polymer	960	109267.9	1	UniProt (Q9Y2L1) Pfam (PF13638) Pfam (PF17216) Pfam (PF17849) Pfam (PF00773) Pfam (PF17215) In PDB	Homo sapiens (Human)	DIS3,Protein DIS3 homolog,Ribosomal RNA-processing protein 44
12	L	M-phase phosphoprotein 6	polymer	162	19268.0	1	UniProt (Q99547) Pfam (PF10175) In PDB	Homo sapiens (Human)	MPP6, MPHOSPH6
13	M	Exosome RNA helicase MTR4	polymer	1045	118225.0	1	UniProt (P42285) Pfam (PF00270) Pfam (PF00271) Pfam (PF21408) Pfam (PF13234) Pfam (PF08148) In PDB	Homo sapiens (Human)	MTR4, MTREX,ATP-dependent RNA helicase DOB1,ATP-dependent RNA helicase SKIV2L2,Superkiller viralicidic activity 2-like 2,TRAMP-like complex helicase
14	N	RNA (5'-R(*AP*GP*CP*AP*CP*CP*GP*UP*AP*AP*AP*GP*AP*CP*GP*C)-3')	polymer	16	5143.2	1		synthetic construct
15	O	DNA/RNA (62-MER)	polymer	62	19487.1	1		synthetic construct
16	K	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
17	K	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
18	M	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)

Sequence modifications

A: 1 - 456 (UniProt: Q06265)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Ser -6	-	expression tag
Gln -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Asn -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: 0 - 244 (UniProt: Q9NPD3)

PDB	External Database	Details
Met -4	-	expression tag
Ala -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag

C: 1 - 276 (UniProt: Q96B26)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag

D: 1 - 235 (UniProt: Q9NQT4)

PDB	External Database	Details
Ser -1	-	expression tag
Leu 0	-	expression tag

E: 1 - 291 (UniProt: Q15024)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag

G: 1 - 275 (UniProt: Q9NQT5)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag
His 225	Tyr 225	variant

H: 1 - 293 (UniProt: Q13868)

PDB	External Database	Details
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

I: 1 - 195 (UniProt: Q9Y3B2)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag

J: 1 - 648 (UniProt: Q01780)

PDB	External Database	Details
Ser 0	-	expression tag
Asn 313	Asp 313	engineered mutation

J: 661 - 760 (UniProt: Q01780)

PDB	External Database	Details
Ser 649	-	linker
Arg 650	-	linker
Gly 651	-	linker
Ser 652	-	linker
Gly 653	-	linker
Ser 654	-	linker
Gly 655	-	linker
Ser 656	-	linker
Gly 657	-	linker
Ser 658	-	linker
Gly 659	-	linker
Ser 660	-	linker

K: 1 - 958 (UniProt: Q9Y2L1)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 146	Asp 146	engineered mutation
Ser 269	Asn 269	variant
Asn 487	Asp 487	engineered mutation
Asn 843	Lys 843	variant

L: 1 - 160 (UniProt: Q99547)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag

M: 1 - 1042 (UniProt: P42285)

PDB	External Database	Details
Ser -2	-	expression tag
Gly -1	-	expression tag
Asp 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	638582.9
Non-Polymers*	Number of molecules	3
	Total formula weight	595.9
All*	Total formula weight	639178.8

*Water molecules are not included.