6D6Q
Human nuclear exosome-MTR4 RNA complex - overall reconstruction
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.45 |
| Number of Particle | 278185 |
| Software | PHENIX,cryoSPARC,RELION,Leginon,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
