6B73

Crystal Structure of a nanobody-stabilized active state of the kappa-opioid receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Soluble cytochrome b562, kappa-type opioid receptor	polymer	418	47030.0	2	UniProt (P0ABE7) UniProt (P41145) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,KOR-1
2	C, D	Nanobody	polymer	134	14913.3	2		Lama glama
3	A, B	N-[(5alpha,6beta)-17-(cyclopropylmethyl)-3-hydroxy-7,8-didehydro-4,5-epoxymorphinan-6-yl]-3-iodobenzamide	non-polymer		554.4	2	Chemie (CVV)
4	A, B	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
5	A	OLEIC ACID	non-polymer		282.5	1	Chemie (OLA)

Sequence modifications

A, B: -54 - 51 (UniProt: P0ABE7)

PDB	External Database	Details
Gly -59	-	expression tag
Gly -58	-	expression tag
Thr -57	-	expression tag
Thr -56	-	expression tag
Met -55	-	expression tag
Trp -48	Met 29	engineered mutation
Ile 47	His 124	engineered mutation
Leu 51	Arg 128	engineered mutation
Gly 52	-	linker
Ser 53	-	linker

A, B: 54 - 358 (UniProt: P41145)

PDB	External Database	Details
Leu 135	Ile 135	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	123886.7
Non-Polymers*	Number of molecules	5
	Total formula weight	2164.6
All*	Total formula weight	126051.4

*Water molecules are not included.