6B73
Crystal Structure of a nanobody-stabilized active state of the kappa-opioid receptor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Soluble cytochrome b562, kappa-type opioid receptor | polymer | 418 | 47030.0 | 2 | UniProt (P0ABE7) UniProt (P41145) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,KOR-1 |
| 2 | C, D (C, D) | Nanobody | polymer | 134 | 14913.3 | 2 | Lama glama | ||
| 3 | E, H (A, B) | N-[(5alpha,6beta)-17-(cyclopropylmethyl)-3-hydroxy-7,8-didehydro-4,5-epoxymorphinan-6-yl]-3-iodobenzamide | non-polymer | 554.4 | 2 | Chemie (CVV) | |||
| 4 | F, I (A, B) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 5 | G (A) | OLEIC ACID | non-polymer | 282.5 | 1 | Chemie (OLA) |
Sequence modifications
A, B: -54 - 51 (UniProt: P0ABE7)
A, B: 54 - 358 (UniProt: P41145)
| PDB | External Database | Details |
|---|---|---|
| Gly -59 | - | expression tag |
| Gly -58 | - | expression tag |
| Thr -57 | - | expression tag |
| Thr -56 | - | expression tag |
| Met -55 | - | expression tag |
| Trp -48 | Met 29 | engineered mutation |
| Ile 47 | His 124 | engineered mutation |
| Leu 51 | Arg 128 | engineered mutation |
| Gly 52 | - | linker |
| Ser 53 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Leu 135 | Ile 135 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 123886.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 2164.6 | |
| All* | Total formula weight | 126051.4 |
