6B73
Crystal Structure of a nanobody-stabilized active state of the kappa-opioid receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 123 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.310, 150.750, 100.280 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 46.940 - 3.100 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4DJH 5c1m 1m6t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.940 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.187 | 0.961 |
| Number of reflections | 30278 | 4159 |
| <I/σ(I)> | 4 | 0.9 |
| Completeness [%] | 93.6 | 88.2 |
| Redundancy | 3 | 2.5 |
| CC(1/2) | 0.988 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Bis-tris pH 6.5-7.0, 140-200 mM magnesium sulfate hydrate, 100 mM sodium citrate tribasic dehydrate, 10 mM Manganese(II) chloride tetrahydrate, 28-30% PEG400 |
