6APD

Crystal structure of RSV F bound by AM22 and the infant antibody ADI-19425

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Fusion glycoprotein F0,Envelope glycoprotein	polymer	573	63775.9	3	UniProt (C3UPB8) UniProt (M1E1E4) Pfam (PF00523) UniProt (by SIFTS) (P03420) UniProt (by SIFTS) (P10104) In PDB	Respiratory syncytial virus
2	D, F, H	AM22 Fab Heavy Chain,IGH@ protein	polymer	228	24123.2	3	UniProt (Q6GMX6) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)
3	E, G, I	AM22 Fab Light Chain,Uncharacterized protein	polymer	215	23305.0	3	UniProt (Q8TCD0) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens
4	J, K, N	Immunoglobulin heavy variable 3-21,Immunoglobulin gamma-1 heavy chain	polymer	226	23881.7	3	UniProt (A0A0B4J1V1) UniProt (P0DOX5) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
5	L, M, O	IGL@ protein	polymer	218	22955.3	3	UniProt (Q6GMX4) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)

Sequence modifications

A, B, C: 1 - 513 (UniProt: C3UPB8)

PDB	External Database	Details
Glu 66	Lys 66	conflict
Ile 67	Asn 67	engineered mutation
Val 76	Ile 76	conflict
Pro 215	Ser 215	engineered mutation
Ser 514	-	linker
Ala 515	-	linker
Ile 516	-	linker
Gly 517	-	linker

A, B, C: 518 - 546 (UniProt: M1E1E4)

PDB	External Database	Details
Met 547	-	expression tag
Glu 548	-	expression tag
Val 549	-	expression tag
Leu 550	-	expression tag
Phe 551	-	expression tag
Gln 552	-	expression tag
Gly 553	-	expression tag
Pro 554	-	expression tag
Gly 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag
His 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag
Ser 564	-	expression tag
Ala 565	-	expression tag
Trp 566	-	expression tag
Ser 567	-	expression tag
His 568	-	expression tag
Pro 569	-	expression tag
Gln 570	-	expression tag
Phe 571	-	expression tag
Glu 572	-	expression tag
Lys 573	-	expression tag

D, F, H: 1 - 100 (PDB: 6APD)
D, F, H: 101 - 217 (UniProt: Q6GMX6)
E, G, I: 1 - 106 (PDB: 6APD)
E, G, I: 107 - 214 (UniProt: Q8TCD0)
J, K, N: 1 - 94 (UniProt: A0A0B4J1V1)

PDB	External Database	Details
Leu 95	-	linker
Gly 96	-	linker
Tyr 97	-	linker
Cys 98	-	linker
Ser 99	-	linker
Gly 100	-	linker
Gly 100	-	linker
Ser 100	-	linker
Cys 100	-	linker
His 100	-	linker
Phe 100	-	linker
Asp 101	-	linker
Tyr 102	-	linker

J, K, N: 103 - 217 (UniProt: P0DOX5)
L, M, O: 1 - 213 (UniProt: Q6GMX4)

PDB	External Database	Details
Lys 45	Arg 66	conflict
Phe 95	-	insertion
Tyr 96	Val 119	conflict
Val 97	Met 120	conflict
Thr 100	Gly 123	conflict
Val 104	Leu 127	conflict

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	474123.1
All*	Total formula weight	474123.1

*Water molecules are not included.