6APD
Crystal structure of RSV F bound by AM22 and the infant antibody ADI-19425
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 229.470, 229.470, 304.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.958 - 4.100 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.57) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.960 | 50.960 | 4.200 |
| High resolution limit [Å] | 4.100 | 18.790 | 4.100 |
| Rmerge | 0.364 | 0.055 | 1.545 |
| Rmeas | 0.380 | 0.060 | 1.608 |
| Rpim | 0.108 | 0.021 | 0.443 |
| Total number of observations | 785734 | 6056 | 57821 |
| Number of reflections | 64175 | 743 | 4439 |
| <I/σ(I)> | 6.3 | 24.9 | 1.9 |
| Completeness [%] | 99.9 | 94.7 | 100 |
| Redundancy | 12.2 | 8.2 | 13 |
| CC(1/2) | 0.995 | 0.997 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 10% PEG 4000 10% 2-propanol 0.1 M sodium citrate pH 5.5 |
