5ZLN

Crystal structure of mouse TLR9 in complex with two DNAs (CpG DNA and TCGCCA DNA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Toll-like receptor 9	polymer	793	88845.0	2	UniProt (Q9EQU3) Pfam (PF18837) Pfam (PF13855) In PDB	Mus musculus (Mouse)
2	C, D	DNA (5'-D(*AP*GP*GP*CP*GP*TP*TP*TP*TP*T)-3')	polymer	10	3066.0	2		synthetic construct
3	E, F	DNA (5'-D(*TP*CP*GP*C)-3')	polymer	6	1769.2	2		synthetic construct
4	G, H	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	I, J, K, L, M...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
6	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
8		water	water		18.0	601	Chemie (HOH)

Sequence modifications

A, B: 26 - 818 (UniProt: Q9EQU3)

PDB	External Database	Details
Met 310	Leu 310	engineered mutation
Gln 325	Thr 325	engineered mutation
Ser 378	Leu 378	engineered mutation
Gln 495	Asn 495	engineered mutation
Gln 514	Asn 514	engineered mutation
Ala 573	Thr 573	engineered mutation
His 579	Gln 579	engineered mutation
Gln 670	Asn 670	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	187360.3
Branched	Number of molecules	11
	Total formula weight	4992.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1143.9
All*	Total formula weight	193497.0

*Water molecules are not included.