5ZLN

Crystal structure of mouse TLR9 in complex with two DNAs (CpG DNA and TCGCCA DNA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	114.306, 128.141, 156.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.610 - 2.300
R-factor	0.19369
Rwork	0.191
R-free	0.23847
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wpf
RMSD bond length	0.012
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.610	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	102418
<I/σ(I)>	11.4
Completeness [%]	99.8
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20%(w/v) PEG4000, 0.15M Ammonium sulfate, 0.1M HEPES-NaOH pH7.0