5Y7G

Crystal structure of paFAN1 bound to 1nt 5'flap DNA with gap

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Fanconi-associated nuclease 1 homolog	polymer	580	66913.3	3	UniProt (Q9I2N0) Pfam (PF21315) Pfam (PF18081) Pfam (PF08774)	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	pFAN1
2	D, F, H (D, F, H)	DNA (5'-D(P*GP*TP*TP*GP*GP*GP*AP*TP*TP*G)-3')	polymer	10	3131.1	3		synthetic construct
3	E, G, I (E, G, I)	DNA (5'-D(P*GP*AP*AP*TP*GP*TP*GP*TP*GP*TP*CP*TP*CP*AP*AP*TP*CP*CP*CP*AP*AP*CP*TP*T)-3')	polymer	24	7319.7	3		synthetic construct
4	J, K, L (J, L, N)	DNA (5'-D(P*TP*GP*AP*CP*AP*CP*AP*CP*AP*TP*TP*CP*AP*A)-3')	polymer	14	4232.8	3		synthetic construct
5	M, N, O, P, Q (A, B, C)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)

Sequence modifications

A, B, C: 1 - 559 (UniProt: Q9I2N0)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	244790.6
Non-Polymers*	Number of molecules	5
	Total formula weight	200.4
All*	Total formula weight	244990.9

*Water molecules are not included.