5Y7G

Crystal structure of paFAN1 bound to 1nt 5'flap DNA with gap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	140.290, 143.258, 172.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.580 - 3.400
R-factor	0.2241
Rwork	0.221
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r8a
RMSD bond length	0.013
RMSD bond angle	1.426
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1-2155_1069: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.400
Rmerge	0.162	1.913
Rpim		0.575
Number of reflections	46805
<I/σ(I)>	26.9
Completeness [%]	96.6
Redundancy	12
CC(1/2)		0.222

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	0.1M Bis-Tris-Propane (pH7.6), 12%(w/v) PEG 8000, 0.2M Sodium acetate trihydrate, 10mM Calcium chloride dihydrate, 1%(w/v) PEG 3350