5XHS

Crystal structure of SIRT5 complexed with a fluorogenic small-molecule substrate SuBKA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NAD-dependent protein deacylase sirtuin-5, mitochondrial	polymer	288	31453.7	1	UniProt (Q9NXA8) Pfam (PF02146) In PDB	Homo sapiens (Human)	Regulatory protein SIR2 homolog 5,SIR2-like protein 5
2	A	benzyl chlorocarbonate	non-polymer		170.6	1	Chemie (PHQ)
3	A	(2S)-2-azanyl-6-[(4-hydroxy-4-oxo-butanoyl)amino]hexanoic acid	non-polymer		246.3	1	Chemie (SLL)
4	A	7-AMINO-4-METHYL-CHROMEN-2-ONE	non-polymer		175.2	1	Chemie (MCM)
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6		water	water		18.0	137	Chemie (HOH)

Sequence modifications

A: 34 - 302 (UniProt: Q9NXA8)

PDB	External Database	Details
Met 15	-	expression tag
Gly 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Gly 24	-	expression tag
Glu 25	-	expression tag
Asn 26	-	expression tag
Leu 27	-	expression tag
Tyr 28	-	expression tag
Phe 29	-	expression tag
Gln 30	-	expression tag
Gly 31	-	expression tag
Ala 32	-	expression tag
Ser 33	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31453.7
Non-Polymers*	Number of molecules	4
	Total formula weight	657.4
All*	Total formula weight	32111.1

*Water molecules are not included.