5W0U
Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with dCMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (B, A) | Maltose-binding periplasmic protein,Single-stranded DNA cytosine deaminase | polymer | 549 | 61659.9 | 2 | UniProt (P0AEY0) UniProt (Q9GZX7) Pfam (PF01547) Pfam (PF08210) UniProt (by SIFTS) (P0AEX9) | Escherichia coli O157:H7 | MBP,MMBP,Maltodextrin-binding protein,Activation-induced cytidine deaminase,AID,Cytidine aminohydrolase |
| 2 | B (D) | DNA (5'-D(*GP*TP*TP*CP*AP*AP*GP*GP*CP*CP*AP*G)-3') | polymer | 12 | 3687.4 | 1 | Homo sapiens | ||
| 3 | C (G) | DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*TP*GP*AP*AP*C)-3') | polymer | 12 | 3638.4 | 1 | Homo sapiens | ||
| 4 | E, I (B, A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | F, J (B, A) | 2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE | non-polymer | 307.2 | 2 | Chemie (DCM) | |||
| 6 | G, K (B, A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 7 | H (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 8 | L, M (B, A) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
B, A: 2 - 367 (UniProt: P0AEY0)
B, A: 1013 - 1181 (UniProt: Q9GZX7)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 83 | Asp 108 | engineered mutation |
| Ala 84 | Lys 109 | engineered mutation |
| Ala 173 | Glu 198 | engineered mutation |
| Ala 174 | Asn 199 | engineered mutation |
| Ala 240 | Lys 265 | engineered mutation |
| Ala 360 | Glu 385 | engineered mutation |
| Ala 363 | Lys 388 | engineered mutation |
| Ala 364 | Asp 389 | engineered mutation |
| Asn 368 | - | linker |
| Ala 369 | - | linker |
| Ala 370 | - | linker |
| Ala 371 | - | linker |
| Glu 372 | - | linker |
| Phe 373 | - | linker |
| Met 1006 | - | linker |
| Asp 1007 | - | linker |
| Pro 1008 | - | linker |
| Ala 1009 | - | linker |
| Thr 1010 | - | linker |
| Phe 1011 | - | linker |
| Thr 1012 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Glu 1042 | Phe 42 | engineered mutation |
| Ala 1058 | Glu 58 | engineered mutation |
| Ala 1130 | His 130 | engineered mutation |
| Glu 1131 | Arg 131 | engineered mutation |
| Tyr 1141 | Phe 141 | engineered mutation |
| Glu 1145 | Phe 145 | engineered mutation |
| Gln 1181 | Leu 181 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 130645.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 917.5 | |
| All* | Total formula weight | 131563.0 |
