5TZU

Crystal structure of human CD47 ECD bound to Fab of B6H12.2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Light Chain of Fab B6H12.2	polymer	214	23373.9	1		Homo sapiens
2	B (H)	Heavy Chain of Fab B6H12.2	polymer	227	24196.1	1		Homo sapiens
3	C (C)	Leukocyte surface antigen CD47	polymer	129	14764.5	1	UniProt (Q08722) Pfam (PF08204)	Homo sapiens (Human)	Antigenic surface determinant protein OA3,Integrin-associated protein,IAP,Protein MER6
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E (B)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
6	F (D)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
7	G, H (L, H)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
8	I, J, N (H, C)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
9	K, L (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
10	M (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
11	O, P, Q (L, H, C)	water	water		18.0	333	Chemie (HOH)

Sequence modifications

C: 1 - 123 (UniProt: Q08722)

PDB	External Database	Details
Gly 15	Cys 33	engineered mutation
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	62334.5
Branched	Number of molecules	3
	Total formula weight	1921.8
Non-Polymers*	Number of molecules	8
	Total formula weight	903.8
All*	Total formula weight	65160.1

*Water molecules are not included.