5TZU
Crystal structure of human CD47 ECD bound to Fab of B6H12.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.763, 54.527, 83.494 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 37.760 - 2.100 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.757 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.105 | 0.368 |
| Number of reflections | 42025 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 2.4M ammonium sulfate, 0.1M sodium acetate pH 5.5 |
