5TT2

Inactive conformation of engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, D	Cystathionine gamma-lyase	polymer	422	46610.1	2	UniProt (P32929) Pfam (PF01053) In PDB	Homo sapiens (Human)	Cysteine-protein sulfhydrase,Gamma-cystathionase
2	C, D	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)

Sequence modifications

C, D: 21 - 424 (UniProt: P32929)

PDB	External Database	Details
Met 3	-	initiating methionine
Gly 4	-	expression tag
Gly 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Glu 14	-	expression tag
Val 15	-	expression tag
Leu 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Gly 19	-	expression tag
Pro 20	-	expression tag
Asn 78	Glu 59	engineered mutation
Leu 138	Arg 119	engineered mutation
Val 358	Glu 339	engineered mutation
Ile 422	Ser 403	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93220.2
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	93604.5

*Water molecules are not included.