5TT2
Inactive conformation of engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 120.224, 120.224, 125.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.429 - 2.949 |
R-factor | 0.2028 |
Rwork | 0.200 |
R-free | 0.25420 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.703 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.949 |
Rmerge | 0.151 |
Number of reflections | 19542 |
<I/σ(I)> | 7.4 |
Completeness [%] | 97.7 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 100mM MES pH6.5 20% PEG3350 200mM Na/K tartrate 200mM lithium sulfate |