5TDH
The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, H) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40429.1 | 2 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B, E (B, J) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 342 | 37575.1 | 2 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Rat) | G-beta-1,Transducin beta chain 1 |
| 3 | C, F (G, K) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 2 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G-gamma-2,G gamma-I |
| 4 | G, H (A, H) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) |
Sequence modifications
A, H: 1 - 354 (UniProt: P63096)
B, J: 1 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Ala 98 | Ser 98 | conflict |
| Ala 203 | Gly 203 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 171135.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 886.4 | |
| All* | Total formula weight | 172022.2 |
