5TDH

The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, H)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40429.1	2	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B, E (B, J)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	342	37575.1	2	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Rat)	G-beta-1,Transducin beta chain 1
3	C, F (G, K)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	2	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G-gamma-2,G gamma-I
4	G, H (A, H)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)

Sequence modifications

A, H: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 98	Ser 98	conflict
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

B, J: 1 - 340 (UniProt: P54311)

PDB	External Database	Details
Ser -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	171135.8
Non-Polymers*	Number of molecules	2
	Total formula weight	886.4
All*	Total formula weight	172022.2

*Water molecules are not included.