5TDH

The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-12-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	126.009, 54.570, 138.350
Unit cell angles	90.00, 113.10, 90.00

Refinement procedure

Resolution	43.635 - 3.000
R-factor	0.2783
Rwork	0.276
R-free	0.30530
RMSD bond length	0.003
RMSD bond angle	0.727
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	34720
<I/σ(I)>	5.7
Completeness [%]	98.4
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	293	2% v/v Tacsimate (pH 5.0), 0.1 M sodium citrate tribasic dehydrate (pH 5.6), 16% w/v PEG 3350