5TDH

The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005794	cellular_component	Golgi apparatus
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005856	cellular_component	cytoskeleton
A	0005886	cellular_component	plasma membrane
A	0005938	cellular_component	cell cortex
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
A	0007218	biological_process	neuropeptide signaling pathway
A	0010854	molecular_function	adenylate cyclase regulator activity
A	0010855	molecular_function	adenylate cyclase inhibitor activity
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0019001	molecular_function	guanyl nucleotide binding
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
A	0031749	molecular_function	D2 dopamine receptor binding
A	0031821	molecular_function	G protein-coupled serotonin receptor binding
A	0034451	cellular_component	centriolar satellite
A	0034695	biological_process	response to prostaglandin E
A	0036064	cellular_component	ciliary basal body
A	0043434	biological_process	response to peptide hormone
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0046676	biological_process	negative regulation of insulin secretion
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0060236	biological_process	regulation of mitotic spindle organization
A	0070062	cellular_component	extracellular exosome
A	0070098	biological_process	chemokine-mediated signaling pathway
A	0072678	biological_process	T cell migration
A	1901082	biological_process	positive regulation of relaxation of smooth muscle
A	1904322	biological_process	cellular response to forskolin
A	1904778	biological_process	positive regulation of protein localization to cell cortex
B	0001750	cellular_component	photoreceptor outer segment
B	0001917	cellular_component	photoreceptor inner segment
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
B	0007204	biological_process	positive regulation of cytosolic calcium ion concentration
B	0008283	biological_process	cell population proliferation
B	0010659	biological_process	cardiac muscle cell apoptotic process
B	0030159	molecular_function	signaling receptor complex adaptor activity
B	0030425	cellular_component	dendrite
B	0030507	molecular_function	spectrin binding
B	0042622	cellular_component	photoreceptor outer segment membrane
B	0044297	cellular_component	cell body
B	0044877	molecular_function	protein-containing complex binding
B	0045202	cellular_component	synapse
B	0047391	molecular_function	alkylglycerophosphoethanolamine phosphodiesterase activity
B	0050909	biological_process	sensory perception of taste
B	0051020	molecular_function	GTPase binding
B	0060041	biological_process	retina development in camera-type eye
B	0071456	biological_process	cellular response to hypoxia
G	0003924	molecular_function	GTPase activity
G	0005515	molecular_function	protein binding
G	0005834	cellular_component	heterotrimeric G-protein complex
G	0005886	cellular_component	plasma membrane
G	0007165	biological_process	signal transduction
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
G	0016020	cellular_component	membrane
G	0031681	molecular_function	G-protein beta-subunit binding
G	0071380	biological_process	cellular response to prostaglandin E stimulus
G	0071870	biological_process	cellular response to catecholamine stimulus
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0001664	molecular_function	G protein-coupled receptor binding
H	0003924	molecular_function	GTPase activity
H	0003925	molecular_function	G protein activity
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005730	cellular_component	nucleolus
H	0005737	cellular_component	cytoplasm
H	0005765	cellular_component	lysosomal membrane
H	0005794	cellular_component	Golgi apparatus
H	0005813	cellular_component	centrosome
H	0005829	cellular_component	cytosol
H	0005834	cellular_component	heterotrimeric G-protein complex
H	0005856	cellular_component	cytoskeleton
H	0005886	cellular_component	plasma membrane
H	0005938	cellular_component	cell cortex
H	0007165	biological_process	signal transduction
H	0007186	biological_process	G protein-coupled receptor signaling pathway
H	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
H	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
H	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
H	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
H	0007218	biological_process	neuropeptide signaling pathway
H	0010854	molecular_function	adenylate cyclase regulator activity
H	0010855	molecular_function	adenylate cyclase inhibitor activity
H	0016020	cellular_component	membrane
H	0016787	molecular_function	hydrolase activity
H	0019001	molecular_function	guanyl nucleotide binding
H	0019003	molecular_function	GDP binding
H	0030496	cellular_component	midbody
H	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
H	0031749	molecular_function	D2 dopamine receptor binding
H	0031821	molecular_function	G protein-coupled serotonin receptor binding
H	0034451	cellular_component	centriolar satellite
H	0034695	biological_process	response to prostaglandin E
H	0036064	cellular_component	ciliary basal body
H	0043434	biological_process	response to peptide hormone
H	0045542	biological_process	positive regulation of cholesterol biosynthetic process
H	0046676	biological_process	negative regulation of insulin secretion
H	0046872	molecular_function	metal ion binding
H	0051301	biological_process	cell division
H	0060236	biological_process	regulation of mitotic spindle organization
H	0070062	cellular_component	extracellular exosome
H	0070098	biological_process	chemokine-mediated signaling pathway
H	0072678	biological_process	T cell migration
H	1901082	biological_process	positive regulation of relaxation of smooth muscle
H	1904322	biological_process	cellular response to forskolin
H	1904778	biological_process	positive regulation of protein localization to cell cortex
J	0001750	cellular_component	photoreceptor outer segment
J	0001917	cellular_component	photoreceptor inner segment
J	0003924	molecular_function	GTPase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005834	cellular_component	heterotrimeric G-protein complex
J	0005886	cellular_component	plasma membrane
J	0007165	biological_process	signal transduction
J	0007186	biological_process	G protein-coupled receptor signaling pathway
J	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
J	0007204	biological_process	positive regulation of cytosolic calcium ion concentration
J	0008283	biological_process	cell population proliferation
J	0010659	biological_process	cardiac muscle cell apoptotic process
J	0030159	molecular_function	signaling receptor complex adaptor activity
J	0030425	cellular_component	dendrite
J	0030507	molecular_function	spectrin binding
J	0042622	cellular_component	photoreceptor outer segment membrane
J	0044297	cellular_component	cell body
J	0044877	molecular_function	protein-containing complex binding
J	0045202	cellular_component	synapse
J	0047391	molecular_function	alkylglycerophosphoethanolamine phosphodiesterase activity
J	0050909	biological_process	sensory perception of taste
J	0051020	molecular_function	GTPase binding
J	0060041	biological_process	retina development in camera-type eye
J	0071456	biological_process	cellular response to hypoxia
K	0003924	molecular_function	GTPase activity
K	0005515	molecular_function	protein binding
K	0005834	cellular_component	heterotrimeric G-protein complex
K	0005886	cellular_component	plasma membrane
K	0007165	biological_process	signal transduction
K	0007186	biological_process	G protein-coupled receptor signaling pathway
K	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
K	0016020	cellular_component	membrane
K	0031681	molecular_function	G-protein beta-subunit binding
K	0071380	biological_process	cellular response to prostaglandin E stimulus
K	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue GDP A 401

Chain	Residue
A	ALA41
A	LEU175
A	ARG176
A	THR177
A	ASN269
A	LYS270
A	ASP272
A	LEU273
A	CYS325
A	SER326
A	THR327
A	GLY42
A	GLU43
A	SER44
A	GLY45
A	LYS46
A	SER47
A	THR48
A	SER151

---

site_id	AC2
Number of Residues	16
Details	binding site for residue GDP H 401

Chain	Residue
H	ALA41
H	GLY42
H	GLU43
H	SER44
H	GLY45
H	LYS46
H	SER47
H	THR48
H	LEU175
H	ARG176
H	THR177
H	ASN269
H	LYS270
H	ASP272
H	SER326
H	THR327

---

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
B	LEU70-SER84
B	ILE157-ILE171
B	LEU285-ALA299

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7626050

Chain	Residue	Details
G	ALA2
K	ALA2
H	GLU43
H	ASN269

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Cysteine methyl ester => ECO:0000269|PubMed:1903391

Chain	Residue	Details
G	CYS68
K	CYS68
H	SER47
H	THR181

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	LIPID: S-geranylgeranyl cysteine => ECO:0000269|PubMed:12764189, ECO:0000269|PubMed:1903391, ECO:0000269|PubMed:7626050

Chain	Residue	Details
G	CYS68
K	CYS68

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q

Chain	Residue	Details
A	LEU175
H	LEU175

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:21115486

Chain	Residue	Details
A	ASP200
H	ASP200

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q

Chain	Residue	Details
A	SER326
H	SER326

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250

Chain	Residue	Details
A	ARG178
H	ARG178

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269|PubMed:24141704

Chain	Residue	Details
A	GLN204
H	GLN204

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250

Chain	Residue	Details
A	CYS351
H	CYS351

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	LIPID: N-myristoyl glycine => ECO:0000269|PubMed:20213681, ECO:0000269|PubMed:25255805

Chain	Residue	Details
A	GLY2
H	GLY2

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	LIPID: S-palmitoyl cysteine => ECO:0000250|UniProtKB:P10824

Chain	Residue	Details
A	CYS3
H	CYS3

---