5O58
Structure of the inactive T.maritima PDE (TM1595) D80N D154N mutant with substrate 5'-pApG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | phosphodiesterase TM1595 | polymer | 338 | 37868.9 | 1 | UniProt (Q9X1T1) Pfam (PF01368) Pfam (PF02272) | Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) | |
| 2 | B (B) | RNA (5'-R(P*AP*G)-3') | polymer | 2 | 629.5 | 1 | synthetic construct | ||
| 3 | C, D, E, F, G... (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 18 | Chemie (EDO) | |||
| 4 | U, V, W, X, Y (A) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 5 | Chemie (IPA) | |||
| 5 | AA, Z (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 6 | BA, CA (A, B) | water | water | 18.0 | 279 | Chemie (HOH) |
Sequence modifications
A: 1 - 333 (UniProt: Q9X1T1)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Asn 80 | Asp 80 | engineered mutation |
| Asn 154 | Asp 154 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 38498.4 | |
| Non-Polymers* | Number of molecules | 25 |
| Total formula weight | 1488.6 | |
| All* | Total formula weight | 39987.0 |
