5MNJ

Structure of MDM2-MDMX-UbcH5B-ubiquitin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Ubiquitin-conjugating enzyme E2 D2	polymer	147	16851.4	2	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B, F	Polyubiquitin-B	polymer	81	8922.1	2	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
3	C, G	E3 ubiquitin-protein ligase Mdm2	polymer	86	9668.4	2	UniProt (Q00987) Pfam (PF13920) In PDB	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,p53-binding protein Mdm2
4	D, H	Protein Mdm4	polymer	64	7207.7	2	UniProt (O15151) Pfam (PF13920) In PDB	Homo sapiens (Human)	Double minute 4 protein,Mdm2-like p53-binding protein,Protein Mdmx,p53-binding protein Mdm4
5	C, D, G, H	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
6	D, H	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7		water	water		18.0	77	Chemie (HOH)

Sequence modifications

A, E: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

B, F: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, G: 428 - 491 (UniProt: Q00987)

PDB	External Database	Details
Met 406	-	initiating methionine
Gly 407	-	expression tag
Ser 408	-	expression tag
Ser 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
Ser 416	-	expression tag
Gln 417	-	expression tag
Asp 418	-	expression tag
Leu 419	-	expression tag
Glu 420	-	expression tag
Asn 421	-	expression tag
Leu 422	-	expression tag
Tyr 423	-	expression tag
Phe 424	-	expression tag
Gln 425	-	expression tag
Gly 426	-	expression tag
Ser 427	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	85299.3
Non-Polymers*	Number of molecules	10
	Total formula weight	715.4
All*	Total formula weight	86014.7

*Water molecules are not included.