5MNJ
Structure of MDM2-MDMX-UbcH5B-ubiquitin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97879 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.240, 62.760, 66.350 |
| Unit cell angles | 69.83, 69.22, 78.21 |
Refinement procedure
| Resolution | 50.490 - 2.160 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ZNI and 3VJF |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.500 |
| High resolution limit [Å] | 2.160 |
| Number of reflections | 37881 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 95.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M Tris-HCl, pH 8.5, 0.175 M Li2SO4 and 16-20 %(v/v) PEG 3350 |
