5MG3

EM fitted model of bacterial holo-translocon

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (Y)	Protein translocase subunit SecY	polymer	458	50410.5	1	UniProt (P0AGA2) Pfam (PF00344)	Escherichia coli
2	B (E)	Protein translocase subunit SecE	polymer	140	15248.0	1	UniProt (P0AG96) Pfam (PF00584)	Escherichia coli
3	C (G)	Protein-export membrane protein SecG	polymer	136	14326.4	1	UniProt (P0AG99) Pfam (PF03840)	Escherichia coli	P12,Preprotein translocase band 1 subunit
4	D (D)	Protein translocase subunit SecD	polymer	622	67688.0	1	UniProt (P0AG90) Pfam (PF13721) Pfam (PF07549) Pfam (PF21760) Pfam (PF22599) Pfam (PF02355)	Escherichia coli
5	E (F)	Protein translocase subunit SecF	polymer	323	35413.3	1	UniProt (P0AG93) Pfam (PF02355) Pfam (PF07549)	Escherichia coli
6	F (C)	Membrane protein insertase YidC	polymer	559	62658.6	1	UniProt (P25714) Pfam (PF14849) Pfam (PF02096)	Escherichia coli	Foldase YidC,Inner membrane protein YidC,Membrane integrase YidC,Oxa1Ec

Sequence modifications

Y: 1 - 443 (UniProt: P0AGA2)

PDB	External Database	Details
Val -14	-	expression tag
Trp -13	-	expression tag
Asn -12	-	expression tag
Cys -11	-	expression tag
Glu -10	-	expression tag
Arg -9	-	expression tag
Ile -8	-	expression tag
Thr -7	-	expression tag
Ile -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
Arg -3	-	expression tag
Lys -2	-	expression tag
Gln -1	-	expression tag
Thr 0	-	expression tag

E: 384 - 508 (UniProt: P0AG96)

PDB	External Database	Details
Met 369	-	initiating methionine
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
Asp 376	-	expression tag
Asp 377	-	expression tag
Asp 378	-	expression tag
Asp 379	-	expression tag
Lys 380	-	expression tag
Ala 381	-	expression tag
Met 382	-	expression tag
Gly 383	-	expression tag

G: 465 - 574 (UniProt: P0AG99)

PDB	External Database	Details
Val 439	-	expression tag
Gly 440	-	expression tag
Thr 441	-	expression tag
Gly 442	-	expression tag
Trp 443	-	expression tag
Tyr 444	-	expression tag
Ser 445	-	expression tag
Gly 446	-	expression tag
Ser 447	-	expression tag
Pro 448	-	expression tag
Gly 449	-	expression tag
Ile 450	-	expression tag
Leu 451	-	expression tag
Tyr 452	-	expression tag
His 453	-	expression tag
Trp 454	-	expression tag
Pro 455	-	expression tag
Glu 456	-	expression tag
Val 457	-	expression tag
Leu 458	-	expression tag
Arg 459	-	expression tag
Ile 460	-	expression tag
Gln 461	-	expression tag
Glu 462	-	expression tag
Leu 463	-	expression tag
Ile 464	-	expression tag

D: 2 - 615 (UniProt: P0AG90)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Val 142	Ala 142	conflict

C: 2 - 548 (UniProt: P25714)

PDB	External Database	Details
Met -4	-	initiating methionine
Asp -3	-	expression tag
Pro -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Arg 1	-	expression tag
Ala 228	Glu 228	conflict
Ala 229	Lys 229	conflict
Ala 231	Glu 231	conflict
Ala 232	Lys 232	conflict
Ala 234	Lys 234	conflict
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
His 553	-	expression tag
His 554	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	245744.8
All*	Total formula weight	245744.8

*Water molecules are not included.