5MG3
EM fitted model of bacterial holo-translocon
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 100 |
| Detector | FEI FALCON I (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 14 |
| Number of Particle | 84732 |
| Software | EMAN,SPIDER,RELION,EPU,Bsoft |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
