5LUZ

Structure of Human Neurolysin (E475Q) in complex with neurotensin peptide products

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Neurolysin, mitochondrial	polymer	686	78644.8	2	UniProt (Q9BYT8) Pfam (PF01432) In PDB	Homo sapiens (Human)	Angiotensin-binding protein,Microsomal endopeptidase,MEP,Mitochondrial oligopeptidase M,Neurotensin endopeptidase
2	C, D, P, Q	PRO-ARG-ARG-PRO neurotensin fragment	polymer	13	1675.9	4	UniProt (by SIFTS) (P30990) In PDB	Homo sapiens
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	117	Chemie (HOH)

Sequence modifications

A, B: 15 - 681 (UniProt: Q9BYT8)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Gln 475	Glu 498	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163993.4
Non-Polymers*	Number of molecules	5
	Total formula weight	293.8
All*	Total formula weight	164287.2

*Water molecules are not included.