5LUZ
Structure of Human Neurolysin (E475Q) in complex with neurotensin peptide products
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-03 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.968 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 131.329, 131.329, 195.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.21813 |
Rwork | 0.216 |
R-free | 0.26786 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i1i |
RMSD bond length | 0.007 |
RMSD bond angle | 1.068 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.15) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.010 | 0.570 |
Number of reflections | 46686 | |
<I/σ(I)> | 12.2 | 2.9 |
Completeness [%] | 98.6 | 99.2 |
Redundancy | 5.9 | 6.2 |
CC(1/2) | 0.982 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M Bis-Tris-propane, 11% PEG 3350, 10% glycerol, 0.2M potassium thiocyanate |