5K25

Crystal structure of human phosphatase PRL-2 in complex with the ADP-bound Bateman domain of human magnesium transporter CNNM3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein tyrosine phosphatase type IVA 2	polymer	190	21927.3	1	UniProt (Q12974) Pfam (PF00102)	Homo sapiens (Human)	HU-PP-1,OV-1,PTP(CAAXII),Protein-tyrosine phosphatase 4a2,Protein-tyrosine phosphatase of regenerating liver 2,PRL-2
2	B (C)	Metal transporter CNNM3	polymer	156	17762.2	1	UniProt (Q8NE01) Pfam (PF00571)	Homo sapiens (Human)	Ancient conserved domain-containing protein 3,Cyclin-M3
3	C (C)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	D (C)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 1 - 167 (UniProt: Q12974)

PDB	External Database	Details
Met -22	-	initiating methionine
Ser -21	-	expression tag
Tyr -20	-	expression tag
Tyr -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Ser -10	-	expression tag
Thr -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Lys -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Phe -1	-	expression tag
Ala 0	-	expression tag

C: 309 - 452 (UniProt: Q8NE01)

PDB	External Database	Details
Gln 297	-	expression tag
Gly 298	-	expression tag
Pro 299	-	expression tag
Leu 300	-	expression tag
Asn 301	-	expression tag
Met 302	-	expression tag
Ile 303	-	expression tag
Gln 304	-	expression tag
Gly 305	-	expression tag
Val 306	-	expression tag
Leu 307	-	expression tag
Glu 308	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39689.5
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	40116.7

*Water molecules are not included.