5K25
Crystal structure of human phosphatase PRL-2 in complex with the ADP-bound Bateman domain of human magnesium transporter CNNM3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9179 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.152, 124.401, 164.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.710 - 3.050 |
R-factor | 0.2345 |
Rwork | 0.232 |
R-free | 0.28299 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.839 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.066 | 0.476 |
Number of reflections | 10010 | |
<I/σ(I)> | 26.6 | 4.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.54 M sodium citrate, 0.1 M sodium acetate, 10 mM ATP, 2.5 mM AMP |