5I42

Structure of HIV-1 Reverse Transcriptase in complex with a DNA aptamer, AZTTP, and CA(2+) ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT	polymer	555	63875.1	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
2	B, D	HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT	polymer	444	51928.6	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
3	E, F	DNA (38-MER)	polymer	38	11748.5	2		synthetic construct
4	A, C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	A, C	3'-AZIDO-3'-DEOXYTHYMIDINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (AZT)

Sequence modifications

A, C: 1 - 555 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Met -15	-	initiating methionine
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	255104.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1094.5
All*	Total formula weight	256199.1

*Water molecules are not included.