5FOE
Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | GDP-fucose protein O-fucosyltransferase 2,Thrombospondin-1 | polymer | 500 | 57515.2 | 2 | UniProt (Q8WR51) UniProt (P07996) Pfam (PF10250) Pfam (PF00090) | Caenorhabditis elegans | Patterning defective protein 2,Peptide-O-fucosyltransferase 2,O-FucT-2 |
| 2 | C, K (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 3 | D, J (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | E, F, G, H, I... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 10 | Chemie (EDO) | |||
| 5 | Q, R (A, B) | water | water | 18.0 | 531 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 424 (UniProt: Q8WR51)
A, B: 1001 - 1052 (UniProt: P07996)
| PDB | External Database | Details |
|---|---|---|
| Lys 298 | Arg 298 | engineered mutation |
| Lys 299 | Arg 299 | engineered mutation |
| Ser 425 | - | linker |
| Ser 426 | - | linker |
| Ser 979 | - | linker |
| Ser 980 | - | linker |
| Gly 981 | - | linker |
| Gly 982 | - | linker |
| Gly 983 | - | linker |
| Gly 984 | - | linker |
| Ser 985 | - | linker |
| Gly 986 | - | linker |
| Gly 987 | - | linker |
| Gly 988 | - | linker |
| Gly 989 | - | linker |
| Ser 990 | - | linker |
| Gly 991 | - | linker |
| Gly 992 | - | linker |
| Gly 993 | - | linker |
| Gly 994 | - | linker |
| Gly 995 | - | linker |
| Ser 996 | - | linker |
| Ser 997 | - | linker |
| Gly 998 | - | linker |
| Ser 999 | - | linker |
| Ser 1000 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 115030.5 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1949.5 | |
| All* | Total formula weight | 116980.0 |
