5FOE

Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GDP-fucose protein O-fucosyltransferase 2,Thrombospondin-1	polymer	500	57515.2	2	UniProt (Q8WR51) UniProt (P07996) Pfam (PF10250) Pfam (PF00090)	Caenorhabditis elegans	Patterning defective protein 2,Peptide-O-fucosyltransferase 2,O-FucT-2
2	C, K (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
3	D, J (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	E, F, G, H, I... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
5	Q, R (A, B)	water	water		18.0	531	Chemie (HOH)

Sequence modifications

A, B: 1 - 424 (UniProt: Q8WR51)

PDB	External Database	Details
Lys 298	Arg 298	engineered mutation
Lys 299	Arg 299	engineered mutation
Ser 425	-	linker
Ser 426	-	linker
Ser 979	-	linker
Ser 980	-	linker
Gly 981	-	linker
Gly 982	-	linker
Gly 983	-	linker
Gly 984	-	linker
Ser 985	-	linker
Gly 986	-	linker
Gly 987	-	linker
Gly 988	-	linker
Gly 989	-	linker
Ser 990	-	linker
Gly 991	-	linker
Gly 992	-	linker
Gly 993	-	linker
Gly 994	-	linker
Gly 995	-	linker
Ser 996	-	linker
Ser 997	-	linker
Gly 998	-	linker
Ser 999	-	linker
Ser 1000	-	linker

A, B: 1001 - 1052 (UniProt: P07996)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	115030.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1949.5
All*	Total formula weight	116980.0

*Water molecules are not included.