5FOE
Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.026, 67.447, 90.411 |
| Unit cell angles | 90.00, 116.07, 90.00 |
Refinement procedure
| Resolution | 81.210 - 1.980 |
| R-factor | 0.20168 |
| Rwork | 0.200 |
| R-free | 0.24607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ap5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.565 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 283636 | |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
