5FOE

Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0036066	biological_process	protein O-linked fucosylation
A	0046922	molecular_function	peptide-O-fucosyltransferase activity
B	0036066	biological_process	protein O-linked fucosylation
B	0046922	molecular_function	peptide-O-fucosyltransferase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:Q9Y2G5

Chain	Residue	Details
A	GLU52
B	GLU52

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:26854667, ECO:0007744|PDB:5FOE

Chain	Residue	Details
A	GLY51
A	HIS288
A	ASP366
A	THR383
B	GLY51
B	HIS288
B	ASP366
B	THR383

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Essential for catalytic activity => ECO:0000250|UniProtKB:Q9Y2G5

Chain	Residue	Details
A	ASP391
B	ASP391

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:17761667, ECO:0000269|PubMed:26854667

Chain	Residue	Details
A	ASN205
B	ASN205

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: C-linked (Man) tryptophan => ECO:0000269|PubMed:11067851

Chain	Residue	Details
A	TRP1008
B	TRP1008

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: O-linked (Fuc...) serine => ECO:0000269|PubMed:11067851

Chain	Residue	Details
A	SER1017
B	SER1017

---