5CDR

2.65 structure of S.aureus DNA gyrase and artificially nicked DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	DNA gyrase subunit A	polymer	483	54738.5	2	UniProt (Q99XG5) Pfam (PF00521) In PDB	Staphylococcus aureus (strain N315)
2	B, D	DNA gyrase subunit B,DNA gyrase subunit B	polymer	190	21345.3	2	UniProt (P66937) Pfam (PF01751) Pfam (PF00986) In PDB	Staphylococcus aureus (strain N315)
3	E	DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*)-3')	polymer	8	2451.6	1		synthetic construct
4	G	DNA (5'-D(*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3')	polymer	13	3967.6	1		synthetic construct
5	F	DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3')	polymer	20	6135.0	1		synthetic construct
6	A, B, D	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
7	A, C, E, G	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
8		water	water		18.0	644	Chemie (HOH)

Sequence modifications

A, C: 9 - 491 (UniProt: Q99XG5)

PDB	External Database	Details
Phe 123	Tyr 123	engineered mutation

B, D: 417 - 543 (UniProt: P66937)
B, D: 580 - 640 (UniProt: P66937)

PDB	External Database	Details
Thr 544	-	linker
Gly 545	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	164721.6
Non-Polymers*	Number of molecules	9
	Total formula weight	717.4
All*	Total formula weight	165439.0

*Water molecules are not included.