5CDR
2.65 structure of S.aureus DNA gyrase and artificially nicked DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | DNA gyrase subunit A | polymer | 483 | 54738.5 | 2 | UniProt (Q99XG5) Pfam (PF00521) | Staphylococcus aureus (strain N315) | |
| 2 | B, D (B, D) | DNA gyrase subunit B,DNA gyrase subunit B | polymer | 190 | 21345.3 | 2 | UniProt (P66937) Pfam (PF01751) Pfam (PF00986) | Staphylococcus aureus (strain N315) | |
| 3 | E (E) | DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*)-3') | polymer | 8 | 2451.6 | 1 | synthetic construct | ||
| 4 | F (G) | DNA (5'-D(*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') | polymer | 13 | 3967.6 | 1 | synthetic construct | ||
| 5 | G (F) | DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') | polymer | 20 | 6135.0 | 1 | synthetic construct | ||
| 6 | H, K, N (A, B, D) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
| 7 | I, J, L, M, O... (A, C, E, G) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 8 | Q, R, S, T, U... (A, B, C, D, E...) | water | water | 18.0 | 644 | Chemie (HOH) |
Sequence modifications
A, C: 9 - 491 (UniProt: Q99XG5)
B, D: 417 - 543 (UniProt: P66937)
B, D: 580 - 640 (UniProt: P66937)
| PDB | External Database | Details |
|---|---|---|
| Phe 123 | Tyr 123 | engineered mutation |
B, D: 580 - 640 (UniProt: P66937)
| PDB | External Database | Details |
|---|---|---|
| Thr 544 | - | linker |
| Gly 545 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 164721.6 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 717.4 | |
| All* | Total formula weight | 165439.0 |
