5BRR
Michaelis complex of tPA-S195A:PAI-1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (I) | Plasminogen activator inhibitor 1 | polymer | 379 | 42782.0 | 1 | UniProt (P05121) Pfam (PF00079) | Homo sapiens (Human) | PAI-1,Endothelial plasminogen activator inhibitor,Serpin E1 |
| 2 | B (E) | Tissue-type plasminogen activator | polymer | 252 | 28123.8 | 1 | UniProt (P00750) Pfam (PF00089) | Homo sapiens (Human) | tPA |
| 3 | C, D, E, F (E) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | G (E) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 5 | H, I (I, E) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
I: 1 - 379 (UniProt: P05121)
E: 16 - 244 (UniProt: P00750)
| PDB | External Database | Details |
|---|---|---|
| His 150 | Asn 173 | engineered mutation |
| Thr 154 | Lys 177 | engineered mutation |
| Leu 319 | Gln 342 | engineered mutation |
| Ile 354 | Met 377 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 122 | Cys 430 | engineered mutation |
| Gln 173 | Asn 483 | engineered mutation |
| Ala 195 | Ser 513 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 70905.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 518.5 | |
| All* | Total formula weight | 71424.4 |
