5BRR

Michaelis complex of tPA-S195A:PAI-1

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0004252	molecular_function	serine-type endopeptidase activity
E	0006508	biological_process	proteolysis
I	0001525	biological_process	angiogenesis
I	0002020	molecular_function	protease binding
I	0004866	molecular_function	endopeptidase inhibitor activity
I	0004867	molecular_function	serine-type endopeptidase inhibitor activity
I	0005102	molecular_function	signaling receptor binding
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005615	cellular_component	extracellular space
I	0005886	cellular_component	plasma membrane
I	0009617	biological_process	response to bacterium
I	0010757	biological_process	negative regulation of plasminogen activation
I	0014912	biological_process	negative regulation of smooth muscle cell migration
I	0030194	biological_process	positive regulation of blood coagulation
I	0030195	biological_process	negative regulation of blood coagulation
I	0030336	biological_process	negative regulation of cell migration
I	0030414	molecular_function	peptidase inhibitor activity
I	0031012	cellular_component	extracellular matrix
I	0031093	cellular_component	platelet alpha granule lumen
I	0032757	biological_process	positive regulation of interleukin-8 production
I	0033629	biological_process	negative regulation of cell adhesion mediated by integrin
I	0035491	biological_process	positive regulation of leukotriene production involved in inflammatory response
I	0042730	biological_process	fibrinolysis
I	0045766	biological_process	positive regulation of angiogenesis
I	0045861	biological_process	negative regulation of proteolysis
I	0048260	biological_process	positive regulation of receptor-mediated endocytosis
I	0050729	biological_process	positive regulation of inflammatory response
I	0050820	biological_process	positive regulation of coagulation
I	0050829	biological_process	defense response to Gram-negative bacterium
I	0051918	biological_process	negative regulation of fibrinolysis
I	0061044	biological_process	negative regulation of vascular wound healing
I	0061045	biological_process	negative regulation of wound healing
I	0070062	cellular_component	extracellular exosome
I	0070495	biological_process	negative regulation of thrombin-activated receptor signaling pathway
I	0071222	biological_process	cellular response to lipopolysaccharide
I	0090026	biological_process	positive regulation of monocyte chemotaxis
I	0090399	biological_process	replicative senescence
I	0097187	biological_process	dentinogenesis
I	1901331	biological_process	positive regulation of odontoblast differentiation
I	1902042	biological_process	negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
I	2000098	biological_process	negative regulation of smooth muscle cell-matrix adhesion
I	2000352	biological_process	negative regulation of endothelial cell apoptotic process
I	2001045	biological_process	negative regulation of integrin-mediated signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue GOL E 301

Chain	Residue
E	ARG39
E	PHE40
I	PRO349
I	GLU351

---

site_id	AC2
Number of Residues	4
Details	binding site for residue GOL E 302

Chain	Residue
E	GLU135
E	GLU137
E	HIS159
E	LEU202

---

site_id	AC3
Number of Residues	6
Details	binding site for residue GOL E 303

Chain	Residue
E	HIS37
E	ARG37
E	HIS63
E	ASP132
E	TRP133
E	LYS36

---

site_id	AC4
Number of Residues	3
Details	binding site for residue GOL E 304

Chain	Residue
E	GLU38
E	ARG70
E	HOH413

---

site_id	AC5
Number of Residues	7
Details	binding site for residue PGE E 305

Chain	Residue
E	GLY19
E	LEU20
E	PHE21
E	PHE94
E	ASP96
E	HIS144
E	LYS156

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LSAAHC

Chain	Residue	Details
E	LEU53-CYS58

---

site_id	PS00284
Number of Residues	11
Details	SERPIN Serpins signature. IIIDRPFLFvV

Chain	Residue	Details
I	ILE352-VAL362

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Site: {"description":"Reactive bond"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	250
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"8613982","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Site: {"description":"Important for single-chain activity"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","featureId":"CAR_000031","evidences":[{"source":"PubMed","id":"2513186","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 798

Chain

Residue

Details

---