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5BRR

Michaelis complex of tPA-S195A:PAI-1

Functional Information from GO Data
ChainGOidnamespacecontents
E0004252molecular_functionserine-type endopeptidase activity
E0006508biological_processproteolysis
I0001525biological_processangiogenesis
I0002020molecular_functionprotease binding
I0004867molecular_functionserine-type endopeptidase inhibitor activity
I0005102molecular_functionsignaling receptor binding
I0005515molecular_functionprotein binding
I0005576cellular_componentextracellular region
I0005615cellular_componentextracellular space
I0005886cellular_componentplasma membrane
I0010469biological_processregulation of signaling receptor activity
I0010757biological_processnegative regulation of plasminogen activation
I0010951biological_processnegative regulation of endopeptidase activity
I0014912biological_processnegative regulation of smooth muscle cell migration
I0030194biological_processpositive regulation of blood coagulation
I0030195biological_processnegative regulation of blood coagulation
I0030336biological_processnegative regulation of cell migration
I0031093cellular_componentplatelet alpha granule lumen
I0032757biological_processpositive regulation of interleukin-8 production
I0033629biological_processnegative regulation of cell adhesion mediated by integrin
I0035491biological_processpositive regulation of leukotriene production involved in inflammatory response
I0042730biological_processfibrinolysis
I0045766biological_processpositive regulation of angiogenesis
I0048260biological_processpositive regulation of receptor-mediated endocytosis
I0050729biological_processpositive regulation of inflammatory response
I0050829biological_processdefense response to Gram-negative bacterium
I0051918biological_processnegative regulation of fibrinolysis
I0061044biological_processnegative regulation of vascular wound healing
I0061045biological_processnegative regulation of wound healing
I0062023cellular_componentcollagen-containing extracellular matrix
I0070062cellular_componentextracellular exosome
I0071222biological_processcellular response to lipopolysaccharide
I0090026biological_processpositive regulation of monocyte chemotaxis
I0090399biological_processreplicative senescence
I0097180cellular_componentserine protease inhibitor complex
I0097187biological_processdentinogenesis
I1901331biological_processpositive regulation of odontoblast differentiation
I1902042biological_processnegative regulation of extrinsic apoptotic signaling pathway via death domain receptors
I1904090cellular_componentpeptidase inhibitor complex
I2000098biological_processnegative regulation of smooth muscle cell-matrix adhesion
I2000352biological_processnegative regulation of endothelial cell apoptotic process
Functional Information from PDB Data
site_idAC1
Number of Residues4
Detailsbinding site for residue GOL E 301
ChainResidue
EARG39
EPHE40
IPRO349
IGLU351

site_idAC2
Number of Residues4
Detailsbinding site for residue GOL E 302
ChainResidue
EGLU135
EGLU137
EHIS159
ELEU202

site_idAC3
Number of Residues6
Detailsbinding site for residue GOL E 303
ChainResidue
EHIS37
EARG37
EHIS63
EASP132
ETRP133
ELYS36

site_idAC4
Number of Residues3
Detailsbinding site for residue GOL E 304
ChainResidue
EGLU38
EARG70
EHOH413

site_idAC5
Number of Residues7
Detailsbinding site for residue PGE E 305
ChainResidue
EGLY19
ELEU20
EPHE21
EPHE94
EASP96
EHIS144
ELYS156

Functional Information from PROSITE/UniProt
site_idPS00134
Number of Residues6
DetailsTRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LSAAHC
ChainResidueDetails
ELEU53-CYS58

site_idPS00284
Number of Residues11
DetailsSERPIN Serpins signature. IIIDRPFLFvV
ChainResidueDetails
IILE352-VAL362

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3
DetailsACT_SITE: Charge relay system => ECO:0000305|PubMed:8613982
ChainResidueDetails
EHIS57
EASP102
EALA195

site_idSWS_FT_FI2
Number of Residues2
DetailsSITE: Important for single-chain activity
ChainResidueDetails
ELYS156
EASP194

site_idSWS_FT_FI3
Number of Residues1
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:2513186
ChainResidueDetails
EGLN173

Catalytic Information from CSA
site_idMCSA1
Number of Residues4
DetailsM-CSA 798
ChainResidueDetails
EHIS57proton acceptor, proton donor
EASP102electrostatic stabiliser
EGLY193electrostatic stabiliser
EALA195electrostatic stabiliser

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PDB entries from 2024-11-06

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