5BRR
Michaelis complex of tPA-S195A:PAI-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.740, 73.940, 201.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.430 - 3.160 |
R-factor | 0.2071 |
Rwork | 0.203 |
R-free | 0.27596 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.522 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.430 | |
High resolution limit [Å] | 3.160 | 3.160 |
Number of reflections | 13390 | |
<I/σ(I)> | 6.9 | 2.9 |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298.15 | 8% PEG 6000, 0.1 M Tris-HCl |