5BRR
Michaelis complex of tPA-S195A:PAI-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.740, 73.940, 201.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.430 - 3.160 |
| R-factor | 0.2071 |
| Rwork | 0.203 |
| R-free | 0.27596 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.522 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.430 | |
| High resolution limit [Å] | 3.160 | 3.160 |
| Number of reflections | 13390 | |
| <I/σ(I)> | 6.9 | 2.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298.15 | 8% PEG 6000, 0.1 M Tris-HCl |
