4ZUX
SAGA DUB module Ubp8/Sgf11/Sus1/Sgf73 bound to ubiqitinated nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, K, O (A, E, K, O) | Histone H3.2 | polymer | 136 | 15435.1 | 4 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 2 | B, F, L, P (B, F, L, P) | Histone H4 | polymer | 103 | 11394.4 | 4 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G, M, Q (C, G, M, Q) | Histone H2A type 1 | polymer | 130 | 14109.4 | 4 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 4 | D, H, N, R (D, H, N, R) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 4 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I, S (I, S) | DNA (145-MER) | polymer | 145 | 44520.4 | 2 | synthetic construct | ||
| 6 | J, T (J, T) | DNA (145-MER) | polymer | 145 | 44991.7 | 2 | synthetic construct | ||
| 7 | EA, JA, U, Z (U, Z, e, j) | Ubiquitin carboxyl-terminal hydrolase 8 | polymer | 472 | 53731.3 | 4 | UniProt (P50102) Pfam (PF02148) Pfam (PF00443) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Deubiquitinating enzyme 8,Ubiquitin thioesterase 8,Ubiquitin-specific-processing protease 8 |
| 8 | AA, FA, KA, V (V, a, f, k) | Transcription and mRNA export factor SUS1 | polymer | 96 | 11094.5 | 4 | UniProt (Q6WNK7) Pfam (PF10163) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 9 | BA, GA, LA, W (W, b, g, l) | SAGA-associated factor 11 | polymer | 99 | 11297.6 | 4 | UniProt (A6ZWK1) Pfam (PF08209) Pfam (PF18519) UniProt (by SIFTS) (Q03067) | Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) | |
| 10 | CA, HA, MA, X (X, c, h, m) | Polyubiquitin-B | polymer | 76 | 8576.8 | 4 | UniProt (P0CG47) Pfam (PF00240) | Homo sapiens (Human) | |
| 11 | DA, IA, NA, Y (Y, d, i, n) | SAGA-associated factor 73 | polymer | 104 | 11538.1 | 4 | UniProt (P53165) Pfam (PF18508) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | 73 kDa SAGA-associated factor,SAGA histone acetyltransferase complex 73 kDa subunit |
| 12 | AB, BB, CB, DB, EB... (Z, b, d, e, g...) | ZINC ION | non-polymer | 65.4 | 32 | Chemie (ZN) |
Sequence modifications
A, E, K, O: 0 - 135 (UniProt: P84233)
C, G, M, Q: 0 - 129 (UniProt: P06897)
D, H, N, R: 1 - 122 (UniProt: P02281)
U, Z, e, j: 1 - 471 (UniProt: P50102)
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | conflict |
| Ser 123 | Ala 124 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 0 | - | expression tag |
| Ala 146 | Cys 146 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 40 |
| Total formula weight | 782357.1 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 2093.1 | |
| All* | Total formula weight | 784450.2 |
