4ZUX

SAGA DUB module Ubp8/Sgf11/Sus1/Sgf73 bound to ubiqitinated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, K, O	Histone H3.2	polymer	136	15435.1	4	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F, L, P	Histone H4	polymer	103	11394.4	4	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G, M, Q	Histone H2A type 1	polymer	130	14109.4	4	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H, N, R	Histone H2B 1.1	polymer	123	13655.9	4	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I, S	DNA (145-MER)	polymer	145	44520.4	2		synthetic construct
6	J, T	DNA (145-MER)	polymer	145	44991.7	2		synthetic construct
7	U, Z, e, j	Ubiquitin carboxyl-terminal hydrolase 8	polymer	472	53731.3	4	UniProt (P50102) Pfam (PF02148) Pfam (PF00443) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Deubiquitinating enzyme 8,Ubiquitin thioesterase 8,Ubiquitin-specific-processing protease 8
8	V, a, f, k	Transcription and mRNA export factor SUS1	polymer	96	11094.5	4	UniProt (Q6WNK7) Pfam (PF10163) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
9	W, b, g, l	SAGA-associated factor 11	polymer	99	11297.6	4	UniProt (A6ZWK1) Pfam (PF08209) Pfam (PF18519) UniProt (by SIFTS) (Q03067) In PDB	Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
10	X, c, h, m	Polyubiquitin-B	polymer	76	8576.8	4	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
11	Y, d, i, n	SAGA-associated factor 73	polymer	104	11538.1	4	UniProt (P53165) Pfam (PF18508) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	73 kDa SAGA-associated factor,SAGA histone acetyltransferase complex 73 kDa subunit
12	Z, b, d, e, g...	ZINC ION	non-polymer		65.4	32	Chemie (ZN)

Sequence modifications

A, E, K, O: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	conflict

C, G, M, Q: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D, H, N, R: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	conflict

U, Z, e, j: 1 - 471 (UniProt: P50102)

PDB	External Database	Details
Ala 0	-	expression tag
Ala 146	Cys 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	40
	Total formula weight	782357.1
Non-Polymers*	Number of molecules	32
	Total formula weight	2093.1
All*	Total formula weight	784450.2

*Water molecules are not included.